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The Beckman Coulter Cytomics FC500 MCL Flow Cytometer System with UPS is designed to help scientists optimize their workflow efficiency while still maintaining cost effectiveness. This unit provides automated tube based acquisition for cell based assays.


• Uniphase Argon ion, 488nm, 20mW output
• Coherent Red Solid State Diode, 635nm, 25mW output

Flow Cell

150 x 450 micron rectangular channel BioSense enhanced optics, quartz mounted, with vertical (upwards) flow path for superior hydrodynamic focusing.

User-Interchangeable Optical Filters

• Band Pass (BP): 525nm, 575nm, 620nm, 675nm, 755nm
• Dichroic Long Pass (DL): 488nm, 550nm, 600nm, 710nm
• Dichroic Short Pass (DS): 615nm, 645nm
• Long Pass (LP): 500nm
• Short Pass (SP): 620nm, 530nm


• Forward Scatter Detector – Solid state detector with patented Fourier design, providing integrated dual
collection angle selections
• Side Scatter Detector – High performance photodiode
• Fluorescent Detectors – High performance photomultipliers with spectral sensitivity from 185nm-900nm

Flow Rates

Continuous pressure is applied to the sample tube based on
user-selected flow rates.
• Low, Medium and High

Sensitivity and Resolution

• Resolves 0.5µm diameter particles from background noise, with maximum detection up to 40µm diameter particles
• < 600 MESF for FITC*
• < 300 MESF for PE*
• < 600 MESF for APC**

Detector Parameters/Data Acquisition

Up to 16 parameters including Auxiliary, Prism, Time and Ratio can be acquired simultaneously from a possible 24 available parameters.
• Forward Scatter – linear, log and peak
• Side Scatter – linear, log and peak
• PMT1-PMT5- linear, log and peak
• One Auxiliary (AUX) signal assignable to any linear or
peak signal
• Time
• Ratio (derived parameter)
• Four Decade logarithmic Digital Signal Processing (DSP)
of linear FS, SS, FL1-FL5 data

Throughput and Acquisition Rates

Throughput is linked to various items such as the number of events acquired, flow rate, sample concentration, target population, sampling mode and parameters acquired. Maximum acquisition rate is 3,300 events/sec.

Sample Handling

Biohazard contained wash station rinses sample probe preventing carryover.
• 12 x 75mm glass or plastic tubes
• Single tube sampling mode
• 32 tube Multi Carousel Loader (MCL)
• The MCL patented design vortexes each tube individually before sample acquisition
• The MCL uses a visible Class II laser reader operating at 670nm with a maximum power output of 1mW, for use with Interleaved 2-of-5, Code 39, Codabar, Code 128 bar codes.